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What is the difference between pi and ph - jhi

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So, a molar concentration of 10 -7 protons is much easier to read as a pH of 7. However, if you add a stronger acid, like HCl, to the solution, acetic acid will start accepting protons from HCl, and act like a base. The more readily it does this, the more acidic the chemical is. Take the negative log of this result, and you get your pKa. Strong acids, being adept at giving away protons, will tend to have highly dissociated protons and conjugate bases in solution, and therefore have a large Ka.

The pI of a protein is determined by the aggregate pH and therefore pKa of every amino acid in the protein chain. Each amino acid has its own pKa and pI , but can vary according to how many other amino acids are surrounding your target amino acid. Remember, acid behavior can be relative this also shows us why protein structure is crucial to understanding its function! The pI for very simple proteins, like two amino acids, is just the average pKa for each amino acid.

Complex proteins, however, are much more difficult to calculate a pI for. But calculating pI can be important; most proteins carry a slight negative charge at biologically active pH 7. Also, the pI is the point where the protein is least soluble in water because it has no net charge ; this can help determine which proteins are more soluble than others. Asked 7 years, 7 months ago. Active 7 years, 7 months ago. Viewed 5k times. Improve this question. Piesel Piesel 97 2 2 silver badges 7 7 bronze badges.

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